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Membrane filters are thin materials used in chromatography to separate particles from liquids or gases. They work by trapping impurities on the surface or within the pores while allowing the sample to pass through. Common membrane materials include cellulose acetate, PTFE, nylon, and PES, chosen for their chemical compatibility. Membrane filters are widely applied in sample preparation, sterilization, and clarification. Their use ensures cleaner samples, protects equipment, and improves analytical accuracy.